Strong specificity

High sensitivity

Good repeatability

Can be used in cattle, pigs, goats and sheep testing

The reagent is ready to use, reducing errors

Rapid detection of antibodies Yang: vaccine immunization, 14d

Use:

Antibody Evaluation for Type 0 Foot and Mouth Disease Vaccine

Used for the diagnosis of O-type foot-and-mouth disease in un-vaccinated herds

(1) All reagents should be equilibrated at room temperature (25 ± 3 ° C) for 30 minutes or more before use.

(2) Add 80 μL of dilution buffer to each well of the FMDV O-antigen coated plate.

(3) Add 20 μL of sample, positive and negative controls to the wells of the coated plate (plate) containing the dilution buffer at a final dilution ratio of 1: 5.

(4) Cover the membrane to cover the reaction plate and incubate at 25 ± 3 ° C for 60 minutes.

(5) The reaction plate was washed with 1 × washing solution, 300 μL / well, washed 3 times, after the last wash, the remaining liquid in the plate hole was patted dry. Avoid microplate well drying between each wash and before adding the next reagent.

(6) Add 100 μL of HRPO-FMDV Antibody Enzyme Conjugate (Type O) to each well.

(7) Cover plate covers the reaction plate and incubate at 25 ± 3 ° C for 60 minutes.

(8) Repeat step (5).

(9) Add 100 μL of TMB substrate solution to each well.

(10) Cover the membrane cover the reaction plate, incubated at 25 ± 3 ℃ for 15 minutes in the dark.

(11) Add 50 μL stop solution to each well.

(12) OD450nm was measured with a microplate reader.

(13) Calculation results.